Coding

Part:BBa_K3165000:Design

Designed by: Mohit Das   Group: iGEM19_IISc-Bangalore   (2019-10-17)


mini-CcaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For the construction of mini-CcaS, the amino acids between Q221 (which follows the GAF domain) and E-504 to A-514 (linker region upstream of the histidine kinase domain) were deleted to remove the two PAS domains of the protein. The part has been codon optimised for Escherichia coli.

Source

The sequence of this part was obtained from Addgene:pNO286-3, following the work done by Sode and co-workers.

References